Analysis of the MDM2 antagonist nutlin-3 in human prostate cancer cells.

@article{Logan2007AnalysisOT,
  title={Analysis of the MDM2 antagonist nutlin-3 in human prostate cancer cells.},
  author={Ian R Logan and Hesta V. McNeill and Susan Cook and Xiaohong Lu and John Lunec and Craig N. Robson},
  journal={The Prostate},
  year={2007},
  volume={67 8},
  pages={900-6}
}
BACKGROUND Small molecule MDM2 antagonists including nutlin-3 have been shown to be effective against a range of cancer cell types and nutlin-3 can inhibit growth of LNCaP xenografts. We compared the efficacy of nutlin-3 in three prostate cancer cell types and provide an insight into the mechanism of nutlin-3. METHODS Nutlin-3 efficacy was measured using proliferation assays, cell cycle analysis, apoptosis assays, quantitative RT-PCR, and immunoblotting. Chromatin immunoprecipitation (ChIP… CONTINUE READING