Analysis of the C-myc promoter-binding factor zf87/pur1 in transformed and nontransformed cell-lines.

ZF87/Pur1 is a zinc finger protein that binds to the purine-rich element ME1a1 within the c-myc P2 promoter. To better understand the effect of ZF87/Pur1 on c-myc gene expression, the gene was stably expressed in nontransformed NIH3T3 fibroblasts or was transiently overexpressed in transformed COS cells. The protein was targeted almost exclusively to the… CONTINUE READING