Analysis of the Borrelia burgdorferi GeHo fla gene and antigenic characterization of its gene product.

@article{Gassmann1991AnalysisOT,
  title={Analysis of the Borrelia burgdorferi GeHo fla gene and antigenic characterization of its gene product.},
  author={G S Gassmann and Erica Jacobs and Rainer Deutzmann and Ulf Berthold G{\"o}bel},
  journal={Journal of bacteriology},
  year={1991},
  volume={173 4},
  pages={1452-9}
}
The fla gene of Borrelia burgdorferi GeHo was analyzed and expressed in Escherichia coli. The structural gene encodes a flagellar protein of 336 amino acids. Comparative sequence analysis of the amino acid sequence revealed a high degree of sequence conservation with flagellins from both phylogenetically related and unrelated bacteria. The antigenic properties of the B. burgdorferi Fla protein were studied by synthesizing overlapping octapeptides, which were screened by using a battery of… CONTINUE READING