Analysis of temperature-sensitive mutant ts 187 of Saccharomyces cerevisiae altered in a component required for the initiation of protein synthesis.

@article{Feinberg1982AnalysisOT,
  title={Analysis of temperature-sensitive mutant ts 187 of Saccharomyces cerevisiae altered in a component required for the initiation of protein synthesis.},
  author={B J Feinberg and Calvin McLaughlin and Kivie Moldave},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 18},
  pages={10846-51}
}
Postpolysomal extracts have been prepared from wild type haploid Saccharomyces cerevisiae cells (wt A364A) and from a temperature-sensitive mutant strain (ts 187, gene prt 1). The extracts, prepared via spheroplasts and depleted of endogenous mRNA with nuclease, translate exogenous natural mRNA and polyuridylic acid. The activity of wt A364A with respect to… CONTINUE READING