Analysis of telomere lengths in cloned sheep

  title={Analysis of telomere lengths in cloned sheep},
  author={Paul G. Shiels and Alexander J. Kind and Keith Henry Stockman Campbell and D. Waddington and Ian Wilmut and Alan Colman and Angelika Schnieke},
The development of nuclear-transfer techniques using cultured somatic cells allows animals to be produced without involving germline cells. This enables us to examine the importance of the repair of chromosome ends (telomeres) in the germ line and to test the telomere hypothesis of ageing. 
Cloning: questions answered and unsolved.
  • K. Latham
  • Biology
    Differentiation; research in biological diversity
  • 2004
Questions about genome potency, life expectancy of clones, mitochondrial fates, and feasibility of inter-species nuclear transfer are reviewed.
Comparison of T Cell Subsets Between Somatic Cloned and Normal Cow
Developmental problems of somatically cloned animals may cause disorders of the immune system, resulting in the fluctuation of the proportion of white blood cells, different from that of normal animals in peripheral blood.
Nuclear Transfer from Established Cell Lines
The production of cloned animals following nuclear transfer using differentiated somatic cells grown in culture represents a remarkable aspect of developmental biology and future practical applications will require a greater understanding of ‘nuclear reprogramming’.
Ageing: Cloning of mice to six generations
The reiterative cloning of mice to four and six generations in two independent lines shows no signs of prematureageing, and there was no evidence of shortening of telomeres at the ends of chromosomes, normally an indicator of cellular senescence.
Normal telomere lengths found in cloned cattle
The question remains whether Dolly's short telomeres were an exception or a general fact, which would differ from the telomere of fetal-derived clones.
Telomere patterning at the chromosome level in matched donor and reprogrammed cell populations: biology, method development and investigation
This paper aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about concrete mechanical properties of EMTs and its applications in the animal kingdom.
Balancing out the ends during iPSC nuclear reprogramming.
Production efficiency and telomere length of the cloned pigs following serial somatic cell nuclear transfer.
It is suggested that third-generation cloned pigs can be produced by serial somatic cell cloning without compromising production efficiency and that the telomere lengths of cloned pig from the first to third generations are normal.


DNA microsatellite analysis of Dolly
A more detailed microsatellite analysis, which confirms the origin of Dolly, the first animal cloned from an adult mammal, was produced by somatic cell nuclear transfer from a cell population derived from mammary tissue taken from a 6-year-old Finn Dorset ewe.
Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts.
Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk and produced viable animals by nuclear transfer.
Telomeres shorten during ageing of human fibroblasts
The amount and length of telomeric DNA in human fibroblasts does in fact decrease as a function of serial passage during ageing in vitro and possibly in vivo.
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development.
DNA fingerprinting Dolly
A DNA fingerprint analysis is carried out to determine the origin of the donor cell used in nuclear transfer, and has confirmed the authenticity of Dolly.
Work in disorder