Analysis of telomere lengths in cloned sheep

@article{Shiels1999AnalysisOT,
  title={Analysis of telomere lengths in cloned sheep},
  author={Paul G. Shiels and Alexander J. Kind and Keith Henry Stockman Campbell and D. Waddington and Ian Wilmut and Alan Colman and Angelika Schnieke},
  journal={Nature},
  year={1999},
  volume={399},
  pages={316-317}
}
The development of nuclear-transfer techniques using cultured somatic cells allows animals to be produced without involving germline cells. This enables us to examine the importance of the repair of chromosome ends (telomeres) in the germ line and to test the telomere hypothesis of ageing. 
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342 Citations
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  • K. Latham
  • Biology
    Differentiation; research in biological diversity
  • 2004
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Questions about genome potency, life expectancy of clones, mitochondrial fates, and feasibility of inter-species nuclear transfer are reviewed.
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The question remains whether Dolly's short telomeres were an exception or a general fact, which would differ from the telomere of fetal-derived clones.
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TLDR
It is suggested that third-generation cloned pigs can be produced by serial somatic cell cloning without compromising production efficiency and that the telomere lengths of cloned pig from the first to third generations are normal.
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References

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TLDR
A more detailed microsatellite analysis, which confirms the origin of Dolly, the first animal cloned from an adult mammal, was produced by somatic cell nuclear transfer from a cell population derived from mammary tissue taken from a 6-year-old Finn Dorset ewe.
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TLDR
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TLDR
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TLDR
A DNA fingerprint analysis is carried out to determine the origin of the donor cell used in nuclear transfer, and has confirmed the authenticity of Dolly.
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Work in disorder