Analysis of tegumental surface proteins of Schistosoma mansoni primary sporocysts.

@article{Boswell1987AnalysisOT,
  title={Analysis of tegumental surface proteins of Schistosoma mansoni primary sporocysts.},
  author={Carl A. Boswell and Timothy P. Yoshino and Tony Dunn},
  journal={The Journal of parasitology},
  year={1987},
  volume={73 4},
  pages={778-86}
}
Axenically transformed primary sporocysts of Schistosoma mansoni (NMRI strain) were labeled with 125I in an effort to identify sporocyst proteins exposed at the tegumental surface. Using the 125I activating reagent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril, in conjunction with SDS-PAGE and autoradiography, up to 12 bands were radiolabeled out of 60 components visualized by silver staining. Labeled proteins ranged in apparent Mr from greater than 200 to less than 12 kDa. Pronase… CONTINUE READING