Analysis of tear protein patterns of dry-eye patients using fluorescent staining dyes and two-dimensional quantification algorithms.

@article{Grus2001AnalysisOT,
  title={Analysis of tear protein patterns of dry-eye patients using fluorescent staining dyes and two-dimensional quantification algorithms.},
  author={Franz Herman Grus and Perikan Sabuncuo and Albert J. Augustin},
  journal={Electrophoresis},
  year={2001},
  volume={22 9},
  pages={1845-50}
}
Tear proteins of nonstimulated tears of 29 patients (healthy subjects, n = 8; dry-eye syndrome patients, n = 12; diabetic dry-eye patients, n = 9) were electrophoretically separated and stained by SYPRO Orange, followed by Coomassie blue staining. Both, the fluorescent and the Coomassie stains were subsequently analyzed by an automated two-dimensional algorithm for finding and quantification of peaks and by a discriminant analysis. Using SYPRO Orange, an average number of peaks/sample between… CONTINUE READING