Analysis of sugars in glycoproteins by high-pressure liquid chromatography.

@article{Jentoft1985AnalysisOS,
  title={Analysis of sugars in glycoproteins by high-pressure liquid chromatography.},
  author={N H Jentoft},
  journal={Analytical biochemistry},
  year={1985},
  volume={148 2},
  pages={424-33}
}
A method for analyzing the carbohydrate composition of glycoproteins and similar glycoconjugates by methanolysis followed by reverse-phase high-pressure liquid chromatography of the perbenzoylated methyl glycosides has been developed. As described, the method is capable of quantifying sugars in the 1- to 10-nmol range while further optimization of… CONTINUE READING