Analysis of stable oxidized molecular species of glycerophospholipids following treatment of red blood cell ghosts with t-butylhydroperoxide.


A model of lipid peroxidation was employed to investigate the formation of oxidized phospholipids in red blood cell membranes after treatment with t-butylhydroperoxide (tBuOOH). On-line normal-phase HPLC/mass spectrometry (LC/MS) with electrospray ionization was used to separate phospholipid classes and analyze the distribution of the major poly-unsaturated… (More)