Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry.

@article{Graham1990AnalysisOS,
  title={Analysis of sperm cell viability, acrosomal integrity, and mitochondrial function using flow cytometry.},
  author={James K. Graham and Elaine Kunze and Roy H. Hammerstedt},
  journal={Biology of reproduction},
  year={1990},
  volume={43 1},
  pages={
          55-64
        }
}
A triple staining procedure was developed to evaluate bull spermatozoa using flow cytometry. Flow cytometric estimates of cell viability, measured by propidium iodide (PI) exclusion, and acrosomal integrity, measured by Pisum sativum agglutinin (PSA) binding acrosomal contents, were equivalent to estimates made by using standard laboratory assays. Mitochondrial function, measured by rhodamine 123 (R123) fluorescence, was depressed by the mitochondrial inhibitors rotenone (64%) or monensin (52… 
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TLDR
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These techniques are efficient for the simultaneous integrity evaluation of plasma and acrosomal membranes and mitochondrial function in bovine spermatozoa, however, JC-1 has an advantage over MITO and CMXRos, as it separates two cell populations with high and low mitochondrial membrane potential.
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TLDR
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