Analysis of spatial correlations between patterns of DNA damage response and DNA replication in nuclei of cells subjected to replication stress or oxidative damage.

@article{Berna2013AnalysisOS,
  title={Analysis of spatial correlations between patterns of DNA damage response and DNA replication in nuclei of cells subjected to replication stress or oxidative damage.},
  author={Tytus Bernaś and Krzysztof Berniak and Paulina Rybak and Mirosław A Zarebski and Hong Zhao and Zbigniew Darzynkiewicz and Jerzy W. Dobrucki},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2013},
  volume={83 10},
  pages={
          925-32
        }
}
Sites of DNA replication (EdU incorporation) and DNA damage signaling (γH2AX) induced by camptothecin (Cpt) or hydrogen peroxide (H2O2) form characteristic patterns of foci in cell nuclei. The overlap between these patterns is a function of the number of DNA double strand breaks (DSBs) formed in replication sites. The goal of this study was to optimize a method of quantitative assessment of a degree of correlation between these two patterns. Such a correlation can be used to estimate a… CONTINUE READING
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