Analysis of some tryptophan and phenylalanine metabolites in urine by a straight-phase high-performance liquid chromatographic technique.


A high-performance liquid column chromatographic technique is reported for the analysis of some tryptophan and phenylalanine acid metabolites in the urine. An acidified and NaCl-saturated urine sample is loaded on to a C18-bonded silica microcolumn. After washing the microcolumn with clean and deionized water, the metabolites of interest are selectively… (More)


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