Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations.

@article{Hellwig2001AnalysisOS,
  title={Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations.},
  author={Stephan Hellwig and F Emde and Nicole Raven and M. Henke and Pauline van der Logt and Rainer Fischer},
  journal={Biotechnology and bioengineering},
  year={2001},
  volume={74 4},
  pages={344-52}
}
In the last few years the Pichia pastoris expression system has been gaining more and more interest for the expression of recombinant proteins. Many groups have employed fermentation technology in their investigations because the system is fairly easy to scale up and suitable for the production in the milligram to gram range. A large number of heterologous proteins from different sources has been expressed, but the fermentation process technology has been investigated to a lesser extent. A… CONTINUE READING
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