Analysis of short interfering RNA function in RNA interference by using Drosophila embryo extracts and schneider cells.

@article{Lipardi2005AnalysisOS,
  title={Analysis of short interfering RNA function in RNA interference by using Drosophila embryo extracts and schneider cells.},
  author={Concetta Lipardi and Hye Jung Baek and Qin Wei and Bruce M. Paterson},
  journal={Methods in enzymology},
  year={2005},
  volume={392},
  pages={351-71}
}
The realization that short double-stranded RNA (dsRNAs) 21-25 bp in length represent the basis for posttranscriptional gene silencing (PTGS) in plants, quelling in N. crassa, and RNA interference (RNAi) in C. elegans and Drosophila has given insight into one of the most evolutionarily conserved pathways in eukaryotes. dsRNA that arises due to viral infection, transposon mobilization, random insertion of transgenes near active promoters, transcripts from repetitive elements in the genome, or… CONTINUE READING

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