Analysis of regulatory sequences upstream of the E. coli uvrB gene; involvement of the DnaA protein.

@article{Berg1985AnalysisOR,
  title={Analysis of regulatory sequences upstream of the E. coli uvrB gene; involvement of the DnaA protein.},
  author={Elmer van den Berg and R. H. Geerse and Johan Memelink and Roel A. L. Bovenberg and F A Magn{\'e}e and Peter van de Putte},
  journal={Nucleic acids research},
  year={1985},
  volume={13 6},
  pages={1829-40}
}
A region located upstream of the uvrB promoters P1 and P2 was found to cause high plasmid loss when cloned in multicopy vectors. Two sequence elements responsible for this phenomenon were identified by mapping of spontaneous mutations that restore plasmid maintenance: a sequence known to have in vitro promoter activity and a partially overlapping sequence that shows extensive homology to recognition sites for the DnaA protein. Accordingly alterations in the level of DnaA protein in vivo were… CONTINUE READING

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