Analysis of regions essential for the function of chromosomal replicator sequences from Yarrowia lipolytica

Abstract

Two DNA segments exhibiting ARS (autonomously replicating sequence) activity in the dimorphic yeast Yarrowia lipolytica were cloned from its chromosome on an integrative LEU2 plasmid. These ARS segments, designated YlARS1 and YlARS2, conferred on the hybrid plasmids high transformation efficiency and enabled extrachromosomal transmission of the plasmids in… (More)
DOI: 10.1007/BF00279435

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