Analysis of reduced monoclonal antibodies using size exclusion chromatography coupled with mass spectrometry.

@article{Liu2009AnalysisOR,
  title={Analysis of reduced monoclonal antibodies using size exclusion chromatography coupled with mass spectrometry.},
  author={Hongcheng Liu and Georgeen S Gaza-Bulseco and Chris M. Chumsae},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2009},
  volume={20 12},
  pages={2258-64}
}
Size-exclusion chromatography (SEC) has been widely used to detect antibody aggregates, monomer, and fragments. SEC coupled to mass spectrometry has been reported to measure the molecular weights of antibody; antibody conjugates, and antibody light chain and heavy chain. In this study, separation of antibody light chain and heavy chain by SEC and direct coupling to a mass spectrometer was further studied. It was determined that employing mobile phases containing acetonitrile, trifluoroacetic… CONTINUE READING

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