Analysis of receptor oligomerization by FRAP microscopy

@article{Dorsch2009AnalysisOR,
  title={Analysis of receptor oligomerization by FRAP microscopy},
  author={Sandra Dorsch and Karl-Norbert Klotz and Stefan Engelhardt and Martin J. Lohse and Moritz B{\"u}nemann},
  journal={Nature Methods},
  year={2009},
  volume={6},
  pages={225-230}
}
Here we describe an approach to investigate di- or oligomerization of transmembrane receptors in living cells with fluorescence recovery after photobleaching (FRAP). We immobilized a defined fraction of receptors with antibodies and then measured lateral mobility of the nonimmobilized fraction by FRAP. We validated this approach with CD86 and CD28 as monomeric and dimeric reference proteins, respectively. Di- or oligomerization of G protein–coupled receptors is strongly debated. We studied… CONTINUE READING

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application to GPCR oligomerization

  • Maurel, D. et al. Cell-surface protein-protein interaction an FRET, snap-tag technologies
  • Nat. Methods 5, 561–567
  • 2008

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