Analysis of protrusion dynamics in amoeboid cell motility by means of regularized contour flows

@article{Schindler2021AnalysisOP,
  title={Analysis of protrusion dynamics in amoeboid cell motility by means of regularized contour flows},
  author={Daniel Schindler and Ted Moldenhawer and Maike Stange and Valentino Lepro and Carsten Beta and Matthias Holschneider and Wilhelm Huisinga},
  journal={PLoS Computational Biology},
  year={2021},
  volume={17}
}
Amoeboid cell motility is essential for a wide range of biological processes including wound healing, embryonic morphogenesis, and cancer metastasis. It relies on complex dynamical patterns of cell shape changes that pose long-standing challenges to mathematical modeling and raise a need for automated and reproducible approaches to extract quantitative morphological features from image sequences. Here, we introduce a theoretical framework and a computational method for obtaining smooth… 
Density-Dependent Migration Characteristics of Cancer Cells Driven by Pseudopod Interaction
TLDR
Model analysis demonstrated that pseudopods exerting a pulling force on the cell and interacting via increased adhesion at pseudopod tips could explain the experimentally observed increase in speed and persistence with increasing density in HCC38 cells, implying that the density-dependent migratory behavior could be an emergent property of single-cell characteristics without the need for additional mechanisms.
Density-dependent migration characteristics of cancer cells driven by pseudopod coordination
TLDR
Model analysis demonstrated that strong coordination between pseudopods within single cells could explain the experimentally observed increase in speed and persistence with increasing density in HCC38 cells, implying that the density-dependent migratory behavior could be an emergent property of single-cell characteristics without the need for additional mechanisms.

References

SHOWING 1-10 OF 59 REFERENCES
Morphodynamic profiling of protrusion phenotypes.
TLDR
The level set method is introduced as a numerical scheme to reconstruct continuous boundary movement in time-lapse image sequences with finite time sampling and a numerically less expensive method that satisfactorily approximates the definition of protrusion and retraction is described.
Modeling random crawling, membrane deformation and intracellular polarity of motile amoeboid cells
TLDR
A mathematical model of amoeboid motility that combines noisy bistable kinetics with a dynamic phase field for the cell shape and introduces a single parameter for tuning the balance between polarity formation and intracellular noise to capture cell-to-cell variability.
Quantifying lamella dynamics of cultured cells by SACED, a new computer-assisted motion analysis.
TLDR
A computer-assisted stroboscopic method for the continuous observation of cell dynamics (SACED) that allows one to analyze changes in lamellipodia protrusion and ruffle retraction with high resolution in space and time shows that SACED is at least equal to established methods in terms of accuracy.
High Resolution Tracking of Cell Membrane Dynamics in Moving Cells: an Electrifying Approach
TLDR
This work presents its own novel method for tracking cell boundaries of moving cells, the Electrostatic Contour Migration Method (ECMM), as an alternative to the generally accepted level set method (LSM), and discusses general considerations regarding boundary tracking in the context of mathematical modelling.
Spiral actin-polymerization waves can generate amoeboidal cell crawling
TLDR
The impact of cytoskeletal polymerization waves on cell migration using a phase-field approach finds states reminiscent of amoeboidal cell crawling, and new protrusions are seen to emerge from a nucleation process, generating spiral actin waves in the cell interior.
Cell Shape Dynamics: From Waves to Migration
TLDR
The wave-like character of protrusions provides a plausible mechanism for the zig-zagging of pseudopods and for the ability of cells both to swim in viscous fluids and to navigate complex three dimensional topography.
Plasticity of cell migration resulting from mechanochemical coupling
TLDR
A simple two component biochemical reaction-diffusion model based on relaxation oscillators is presented and the importance of coupling signaling events to cell mechanics and morphology is indicated and may be applicable in a wide variety of cell motility systems.
Simultaneous quantification of cell motility and protein-membrane-association using active contours.
We present a new method for the quantification of dynamic changes in fluorescence intensities at the cell membrane of moving cells. It is based on an active contour method for cell-edge detection,
VASP dynamics during lamellipodia protrusion
TLDR
It is shown that VASP not only co-localizes to adhesion sites with the adaptor proteins vinculin and zyxin, but is also recruited to the tips of lamellipodia in amounts that are directly proportional to the rate of protrusion, indicating that VasP may be an adaptor molecule involved in actin-based cell motility.
Local and global measures of shape dynamics.
TLDR
A simple, yet versatile, framework that provides multiple metrics of cell shape and cell shape dynamics is presented that finds that protrusions zigzag, while retractions remain roughly stationary along the boundary.
...
...