Analysis of proteins from membrane-enriched cerebellar preparations by two-dimensional gel electrophoresis and mass spectrometry.

@article{Friso1999AnalysisOP,
  title={Analysis of proteins from membrane-enriched cerebellar preparations by two-dimensional gel electrophoresis and mass spectrometry.},
  author={Giulia Friso and L E Wikstr{\"o}m},
  journal={Electrophoresis},
  year={1999},
  volume={20 4-5},
  pages={917-27}
}
Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry is a powerful combination for the separation of complex protein mixtures in biological samples and the subsequent identification of individual polypeptides. We have used this approach to construct a database of proteins of the porcine cerebellum, with emphasis on membrane-bound proteins, as part of our studies on the structure and function of the central nervous system. We compared the ability of different solubilization… CONTINUE READING

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