Analysis of protein-protein proximities using the split-ubiquitin system.

@article{Lehming2002AnalysisOP,
  title={Analysis of protein-protein proximities using the split-ubiquitin system.},
  author={Norbert Lehming},
  journal={Briefings in functional genomics & proteomics},
  year={2002},
  volume={1 3},
  pages={230-8}
}
  • Norbert Lehming
  • Published 2002 in Briefings in functional genomics & proteomics
The split-ubiquitin system is a fragment complementation assay that is based on a conditional proteolysis design. The system has been used to study protein-protein interactions between membrane proteins and to screen for new interaction partners for transcription factors. This paper outlines the recent progress in the split-ubiquitin technology that has been made possible through the development of new reporter proteins.