Analysis of protein interactions in situ by proximity ligation assays.

@article{Koos2014AnalysisOP,
  title={Analysis of protein interactions in situ by proximity ligation assays.},
  author={B. Koos and L. Andersson and Carl-Magnus Clausson and Karin Grannas and Axel Klaesson and Ga{\"e}lle Cane and O. S{\"o}derberg},
  journal={Current topics in microbiology and immunology},
  year={2014},
  volume={377},
  pages={
          111-26
        }
}
The fate of the cell is governed by interactions among proteins, nucleic acids, and other biomolecules. It is vital to look at these interactions in a cellular environment if we want to increase our understanding of cellular processes. Herein we will describe how the in situ proximity ligation assay (in situ PLA) can be used to visualize protein interactions in fixed cells and tissues. In situ PLA is a novel technique that uses DNA, together with DNA modifying processes such as ligation… Expand
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References

SHOWING 1-10 OF 46 REFERENCES
Direct observation of individual endogenous protein complexes in situ by proximity ligation
  • 1,888
  • PDF
In Situ Proximity Ligation Assay for Microscopy and Flow Cytometry
  • 57
In vitro analysis of DNA–protein interactions by proximity ligation
  • 72
  • PDF
Flow cytometric in situ proximity ligation analyses of protein interactions and post‐translational modification of the epidermal growth factor receptor family
  • 70
Proximity ligation assays: a recent addition to the proteomics toolbox
  • 261
Quantifying RNA–protein interactions in situ using modified-MTRIPs and proximity ligation
  • 43
  • PDF
Visualising individual sequence-specific protein-DNA interactions in situ.
  • 46
  • PDF
Parallel Visualization of Multiple Protein Complexes in Individual Cells in Tumor Tissue*
  • 56
...
1
2
3
4
5
...