Analysis of protein arginine methylation and protein arginine-methyltransferase activity.

Abstract

Posttranslational modification of proteins allows cells to adapt and react quickly to their environment beyond the boundaries set forth by genetic code. Arginine methylation, a protein modification discovered almost 30 years ago, has recently experienced a renewed interest as several new arginine methyltransferases have been identified and numerous proteins were found to be regulated by methylation on arginine residues. Until recently, the detection of arginine methylation required the use of chromatography and mass-spectrometrical analysis. The following protocol provides guidelines for the straightforward identification of arginine-methylated proteins, made possible by the availability of novel, commercially available reagents.

Statistics

0100200'04'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

80 Citations

Semantic Scholar estimates that this publication has 80 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Mowen2001AnalysisOP, title={Analysis of protein arginine methylation and protein arginine-methyltransferase activity.}, author={Kerri A. Mowen and Maria Manuela David}, journal={Science's STKE : signal transduction knowledge environment}, year={2001}, volume={2001 93}, pages={pl1} }