Analysis of primaquine and its metabolite carboxyprimaquine in biological samples: enantiomeric separation, method validation and quantification.

@article{Avula2011AnalysisOP,
  title={Analysis of primaquine and its metabolite carboxyprimaquine in biological samples: enantiomeric separation, method validation and quantification.},
  author={Bharathi Avula and Shabana Iqrar Khan and Babu L. Tekwani and N. P. Dhammika Nanayakkara and James D. Mcchesney and Larry A Walker and Ikhlas Ahmad Khan},
  journal={Biomedical chromatography : BMC},
  year={2011},
  volume={25 9},
  pages={1010-7}
}
The clinical formulation of primaquine (PQ) is a mixture of (-)-(R)- and (+)-(S)- primaquine enantiomers which may show different pharmacokinetic and pharmacodynamic properties. To assess the efficacy and toxicity of primaquine enantiomers, a method using LC-MSD-TOF has been developed. The enantiomers were well separated using a Chiralcel OD column (250 × 4.6 mm, 10 µm) with a linear gradient of mobile phase consisting of acetonitrile (0.1% formic acid) and aqueous ammonium formate (20 mm; 0.1… CONTINUE READING