Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets.

@article{Mayor2005AnalysisOP,
  title={Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets.},
  author={Thibault Mayor and J. Russell Lipford and Johannes Graumann and Geoffrey T. Smith and Raymond J Deshaies},
  journal={Molecular & cellular proteomics : MCP},
  year={2005},
  volume={4 6},
  pages={741-51}
}
The polyubiquitin receptor Rpn10 targets ubiquitylated Sic1 to the 26S proteasome for degradation. In contrast, turnover of at least one ubiquitin-proteasome system (UPS) substrate, CPY*, is impervious to deletion of RPN10. To distinguish whether RPN10 is involved in the turnover of only a small set of cell cycle regulators that includes Sic1 or plays a more general role in the UPS, we sought to develop a general method that would allow us to survey the spectrum of ubiquitylated proteins that… CONTINUE READING