Analysis of polyoma virus nuclear RNA by mini-blot hybridization.


The size and sequence composition of virus-specific RNA extracted from the nuclei of mouse cells late during polyoma virus productive infection were studied by blot-hybridization analysis of 32P-labeled RNA fractionated on CH3HgOH/agarose gels. Viral RNA molecules between approximately 0.4 and 4 times the length of a complete transcript of the 5.4-kilobase… (More)


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@article{Birg1977AnalysisOP, title={Analysis of polyoma virus nuclear RNA by mini-blot hybridization.}, author={Françoise Birg and James W C Favaloro and Robert Kamen}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1977}, volume={74 8}, pages={3138-42} }