Analysis of plants regenerated from protoplast fusions between Brassica napus and Eruca sativa

@article{Fahleson1988AnalysisOP,
  title={Analysis of plants regenerated from protoplast fusions between Brassica napus and Eruca sativa},
  author={Jan Fahleson and L. R{\aa}hl{\'e}n and Kristina Glimelius},
  journal={Theoretical and Applied Genetics},
  year={1988},
  volume={76},
  pages={507-512}
}
Protoplasts from etiolated hypocotyls of Brassica napus stained with carboxyfluorescein were fused with mesophyll protoplasts from Eruca sativa. Hybrid cells could be identified under the light microscope by (1) fully developed chloroplasts derived from E. sativa and (2) the cytoplasmic strands of the B. napus hypocotyl protoplasts, or (3) by the presence of both red and green fluorescence when investigated under UV light. The heterokaryons were selected using either a micro-manipulator or a… CONTINUE READING