Analysis of plant sterol and stanol esters in cholesterol-lowering spreads and beverages using high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy.

@article{Mezine2003AnalysisOP,
  title={Analysis of plant sterol and stanol esters in cholesterol-lowering spreads and beverages using high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy.},
  author={Igor Mezine and Hui-Juan Zhang and Carlos Macku and Robert C. Lijana},
  journal={Journal of agricultural and food chemistry},
  year={2003},
  volume={51 19},
  pages={
          5639-46
        }
}
Plant sterol and stanol esters were separated on a Luna hexyl-phenyl column using a gradient of acetonitrile (90-100%) in water. The eluted compounds were detected by atmospheric pressure chemical ionization (APCI)-mass spectroscopy (MS) in the positive mode. Sterol and stanol esters produced [M + H - HOOCR](+) ions. Application of the hyphenated technique-LC-MS-allowed differentiation between a number of esters of sitosterol, campesterol, stigmasterol, and (tentatively) avenasterol, as well as… 
Gas chromatographic separation of fatty acid esters of cholesterol and phytosterols on an ionic liquid capillary column.
  • S. Hammann, W. Vetter
  • Chemistry, Medicine
    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2015
Analysis of phytostanyl fatty acid esters in enriched foods via UHPLC-APCI-MS.
TLDR
The developed methodology extends the analytical basis for authenticity and quality assessments of functional foods enriched with phytostanyl fatty acid esters to include cholesterol-lowering enriched foods reflecting the diversity in potential matrices.
LC–MS Determination of Sterols in Olive Oil
Sterols in olive oils have been analyzed by liquid chromatography coupled to mass spectrometry with atmospheric-pressure chemical ionization in positive-ion mode. A simple procedure based on
GC-based analysis of plant stanyl fatty acid esters in enriched foods.
TLDR
The robust and rapid methods allow conclusions on both, the stanol profiles and the fatty acid moieties, and thus provide a basis for the authentication of this type of functional food ingredients.
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