Analysis of physiological amino acids using dabsyl derivatization and reversed-phase liquid chromatography.

@article{Drnevich1993AnalysisOP,
  title={Analysis of physiological amino acids using dabsyl derivatization and reversed-phase liquid chromatography.},
  author={D Drnevich and Thomas C. Vary},
  journal={Journal of chromatography},
  year={1993},
  volume={613 1},
  pages={137-44}
}
A method is described for the measurement of the specific radioactivity of primary amino acids in physiological samples. The amino acids were dabsylated followed by separation using high-performance liquid chromatography. We measured the concentration of amino acids from rat plasma or liver samples. Chromatographic analyses resolved phenylalanine from a mixture of amino acids in plasma within 30 min. An extended chromatographic gradient program completely separated all physiological amino acids… CONTINUE READING

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