Analysis of photobleaching in single-molecule multicolor excitation and Förster resonance energy transfer measurements.

  title={Analysis of photobleaching in single-molecule multicolor excitation and F{\"o}rster resonance energy transfer measurements.},
  author={Christian Eggeling and Jerker Widengren and Leif Brand and Joerg Schaffer and Suren Felekyan and Claus A M Seidel},
  journal={The journal of physical chemistry. A},
  volume={110 9},
Dye photobleaching is a major constraint of fluorescence readout within a range of applications. In this study, we investigated the influence of photobleaching in fluorescence experiments applying multicolor laser as well as Förster resonance energy transfer (FRET) mediated excitation using several red-emitting dyes frequently used in multicolor experiments or as FRET acceptors. The chosen dyes (cyanine 5 (Cy5), MR121, Alexa660, Alexa680, Atto647N, Atto655) have chemically distinct chromophore… CONTINUE READING
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