Analysis of pectate lyases produced by soft rot bacteria associated with spoilage of vegetables.

@article{Liao1989AnalysisOP,
  title={Analysis of pectate lyases produced by soft rot bacteria associated with spoilage of vegetables.},
  author={C H Liao},
  journal={Applied and environmental microbiology},
  year={1989},
  volume={55 7},
  pages={1677-83}
}
  • C H Liao
  • Published 1989 in Applied and environmental microbiology
Isoelectric focusing (IEF) profiles of pectate lyases (PLs) produced by five different groups of soft rot bacteria were analyzed by using the combined techniques of thin-layer polyacrylamide gel IEF and agarose-pectate overlay activity staining. Four strains of soft rot Erwinia spp. produced three or more PL isozymes. All of eight Pseudomonas viridiflava strains examined produced one single PL with a pI of 9.7. All 10 of Pseudomonas fluorescens strains produced two PLs; the major one had a pI… CONTINUE READING