Analysis of pancreatic development using a cell lineage label.

  title={Analysis of pancreatic development using a cell lineage label.},
  author={A C Percival and Jonathan Mw Slack},
  journal={Experimental cell research},
  volume={247 1},
We have devised a new culture system for in vitro culture of pancreatic buds from mouse embryos which enables the organ to grow as a flat branched structure suitable for wholemount immunostaining. This system has been used to analyze pancreatic development. We have also used the ROSA-26 gene trap mouse strain as a source of tissue which expresses lacZ in a stable manner, in all cell types, during in vitro culture. Combinations of lacZ epithelium and unlabeled mesenchyme show that both exocrine… CONTINUE READING

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