Analysis of nucleotides by capillary electrophoresis.

  title={Analysis of nucleotides by capillary electrophoresis.},
  author={Susan E Geldart and Phyllis R. Brown},
  journal={Journal of chromatography. A},
  volume={828 1-2},
Capillary electrophoresis is a useful tool for the analysis of nucleotides. Methods have been optimized for both CZE and MECC modes. A variety of CZE buffers, such as borate, carbonate and phosphate were used successfully. The pH of the buffer changes the charge on the nucleotides. Therefore, the selectivity of the analytes can be controlled by the acidity of the buffer solution. CE separations of nucleotides have been performed at all pH levels, in both CZE and MECC modes. SDS was the most… CONTINUE READING