Analysis of nucleotide sugars from cell lysates by ion-pair solid-phase extraction and reversed-phase high-performance liquid chromatography

@article{Rbin2001AnalysisON,
  title={Analysis of nucleotide sugars from cell lysates by ion-pair solid-phase extraction and reversed-phase high-performance liquid chromatography},
  author={Jarkko R{\"a}bin{\"a} and Minna M{\"a}ki and Emma Merike Savilahti and Nina J{\"a}rvinen and Leena Penttil{\"a} and Risto L O Renkonen},
  journal={Glycoconjugate Journal},
  year={2001},
  volume={18},
  pages={799-805}
}
Analysis of nucleotide sugar metabolism is essential in studying glycosylation in cells. Here we describe practical methods for both extraction of nucleotide sugars from cell lysates and for their analytical separation. Solid-phase extraction cartridges containing graphitized carbon can be used for the purification of nucleotide sugars by using triethylammonium acetate buffer as a ion-pairing reagent for decreasing retention. After that they are separated by high-performance liquid… CONTINUE READING

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