Analysis of nucleotide sequence-dependent DNA binding of poly(ADP-ribose) polymerase in a purified system.

@article{Huang2004AnalysisON,
  title={Analysis of nucleotide sequence-dependent DNA binding of poly(ADP-ribose) polymerase in a purified system.},
  author={Kai Huang and William E. Tidyman and K T L{\^e} and Eva Kirsten and Ernest Kun and C. P. Ordahl},
  journal={Biochemistry},
  year={2004},
  volume={43 1},
  pages={217-23}
}
The enzymatic transfer of ADP-ribose from NAD to histone H(1) [defined as trans(oligo-ADP-ribosylation)] or to PARP-1 [defined as auto(poly-ADP-ribosylation)] requires binding of coenzymic DNA. The preceding paper [Kun, E., et al. (2004) Biochemistry 43, 210-216] shows that oligonucleotides of dsDNA can serve as coenzymic DNA for PARP-1 trans- or auto-modification activity. Results of DNA-protein binding (EMSA) experiments reported here demonstrate that short DNA oligonucleotides containing the… CONTINUE READING
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