Analysis of nifH gene pool complexity in soil and litter at a Douglas fir forest site in the Oregon cascade mountain range.

@article{Widmer1999AnalysisON,
  title={Analysis of nifH gene pool complexity in soil and litter at a Douglas fir forest site in the Oregon cascade mountain range.},
  author={Franco Widmer and Brenda T. Shaffer and L. Arlene Porteous and Ramon J. Seidler},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 2},
  pages={374-80}
}
Nitrogen-fixing microbial populations in a Douglas fir forest on the western slope of the Oregon Cascade Mountain Range were analyzed. The complexity of the nifH gene pool (nifH is the marker gene which encodes nitrogenase reductase) was assessed by performing nested PCR with bulk DNA extracted from plant litter and soil. The restriction fragment length polymorphisms (RFLPs) of PCR products obtained from litter were reproducibly different than the RFLPs of PCR products obtained from the… CONTINUE READING
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