Analysis of mutations made during active synthesis or extension of mismatched substrates further define the mechanism of HIV-RT mutagenesis.

@article{Liesch2003AnalysisOM,
  title={Analysis of mutations made during active synthesis or extension of mismatched substrates further define the mechanism of HIV-RT mutagenesis.},
  author={Gwyneth R Liesch and Jeffrey J. DeStefano},
  journal={Biochemistry},
  year={2003},
  volume={42 19},
  pages={
          5925-36
        }
}
The effect of reverse transcriptase (RT) catalyzed mutations on continued extension of the nascent DNA chain was investigated. A system using the alpha-lac gene of beta-galactosidase as template and two sets of conditions was used. In one, RT was allowed to reassociate with the primer-template after falling off, while in a second RT was sequestered after dissociating. In the first condition, subsequent extension of errors that may have initially caused enzyme dissociation can occur. In the… CONTINUE READING

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