Analysis of minocycline by high-performance liquid chromatography in tissue and serum.

  title={Analysis of minocycline by high-performance liquid chromatography in tissue and serum.},
  author={W. Wrightson and S. Myers and S. Galandiuk},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  volume={706 2},
A sensitive and rapid reversed-phase high-performance liquid chromatography assay can be used to accurately determine serum and tissue minocycline concentrations. Minocycline is a broad spectrum tetracycline derivative with many applications. Tissue and serum samples were obtained from guinea pigs that had received either topical or intravenous minocycline. Samples were extracted using a Sep-Pak C18 cartridge and were injected into a microBondapak C18 column with an isocratic methanol mobile… Expand
Liquid chromatographic determination of minocycline in brain-to-plasma distribution studies in the rat.
  • M. Čolović, S. Caccia
  • Chemistry, Medicine
  • Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2003
In the rat minocycline crossed the blood-brain barrier slowly, achieving mean brain concentrations between 30 and 40% of the equivalent systemic exposure, regardless of the dose and route of administration. Expand
Analysis of a second-generation tetracycline antibiotic minocycline in human plasma by LC-MS/MS.
The method was successfully applied to a bioequivalence study of 135 mg minocycline tablet formulation in 14 healthy Indian males and the reproducibility in the measurement of study data was demonstrated by incurred sample reanalysis. Expand
Method Development and Validation for Assay of Minocycline Hydrochloride in Dosage Forms by RP-HPLC
A simple, fast, precise and accurate high performance liquid chromatographic method has been developed for the quantitative estimation of minocycline hydrochloride from powder for oral suspensionExpand
Determination of methacycline in human plasma by liquid chromatography coupled to tandem mass spectrometry
Abstract A rapid, sensitive and selective LC–MS–MS method has been developed and validated for the determination of methacycline in human plasma and employed in a bioequivalence study of two 300 mgExpand
Easy-Assessment of Levofloxacin and Minocycline in Relevant Biomimetic Media by HPLC-UV Analysis.
Simple, economic and environmental friendly high-performance liquid chromatography methods for levofloxacin and minocycline quantification in biomimetic media were developed and validate including their stability at body temperature, an often neglected evaluation parameter, to monitor the in vitro release studies of these two antibiotics. Expand
Validated stability-indicating densitometric thin-layer chromatography: application to stress degradation studies of minocycline.
The acidic and alkaline degradation kinetics of minocycline, evaluated using this method, is found to be of first order. Expand
The results indicated a widespread distribution of minocycline in the tissue samples, which remained within the detectable limit till the 3 rd  day and recommended to pay attention to the proper withdrawal periods before marketing to ensure the hygienic suitability of broilers edibles for safe human consumption. Expand
Development and Validation of an HPLC Confirmatory Method for the Determination of Seven Tetracycline Antibiotics Residues in Bovine and Porcine Muscle Tissues According to 2002/657/EC
Abstract An HPLC method with diode array detection, at 355 nm, was developed and validated for the determination of seven tetracyclines (TCs) in bovine and porcine muscle tissues. ExaminedExpand
Advances in Chromatographic Analysis of Tetracyclines in Foodstuffs of Animal Origin—A Review
Abstract Tetracyclines (TCs) are broad‐spectrum antibiotics in human and veterinary medicine, characterized by their exceptional chemotherapeutic efficacy against a wide range of Gram‐positive andExpand
Liquid Chromatography Based Methods for Analysis of Disease-Modifying Antirheumatic Drugs (DMARDs) in Biological Matrices
This review article can be handy for quantitation of DMARDs in diverse biological matrices as it provides comprehensive information on the reported liquid chromatographic methods for last three decades covering all the aspects required for preclinical and clinical studies. Expand


Determination of tetracyclines in bovine and porcine muscle by high-performance liquid chromatography using solid-phase extraction.
The principal steps involve homogenizing the sample in EDTA-McIlvaine buffer followed by centrifugation and precipitation of the supernatant using trichloroacetic acid, and the analytes are detectable at levels down to 10 ng/g. Expand
A high performance liquid chromatographic system for the analysis of tetracycline drug standards, analogs, degradation products and other impurities.
This paper describes the high performance liquid chromatographic (HPLC) analysis of eight parent tetracycline standards and produced at least partial resolution of all eight parent compounds and many of their degradation products. Expand
A randomized, double-blind trial of single dose piperacillin versus multidose cefoxitin in alimentary tract operations.
This study is among the first of several examining the issue of single dose prophylaxis and will be the harbinger of other studies from other groups examining whether or not the course of therapeutic antibiotics can be safely shortened in patients with peritoneal contamination. Expand