Analysis of microRNA signatures using size-coded ligation-mediated PCR

  title={Analysis of microRNA signatures using size-coded ligation-mediated PCR},
  author={Ehsan Arefian and Jafar Kiani and Masoud Soleimani and Seyed Ali Mohammad Shariati and Seyed Hamid Aghaee-Bakhtiari and Amir Atashi and Yousof Gheisari and Naser Ahmadbeigi and Ali Mohammad Banaei-Moghaddam and Mahmood Naderi and Nabiolah Namvarasl and L. Good and Omid R Faridani},
  booktitle={Nucleic acids research},
The expression pattern and regulatory functions of microRNAs (miRNAs) are intensively investigated in various tissues, cell types and disorders. Differential miRNA expression signatures have been revealed in healthy and unhealthy tissues using high-throughput profiling methods. For further analyses of miRNA signatures in biological samples, we describe here a simple and efficient method to detect multiple miRNAs simultaneously in total RNA. The size-coded ligation-mediated polymerase chain… CONTINUE READING
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