Analysis of messages expressed by Echinostoma paraensei miracidia and sporocysts, obtained by random EST sequencing.

@article{Adema2000AnalysisOM,
  title={Analysis of messages expressed by Echinostoma paraensei miracidia and sporocysts, obtained by random EST sequencing.},
  author={Coen M. Adema and Pascale M. L{\'e}onard and Randall J Dejong and Heather Day and Daniel J Edwards and Galen Burgett and Lynn A Hertel and Eric S. Loker},
  journal={The Journal of parasitology},
  year={2000},
  volume={86 1},
  pages={60-5}
}
A lambdaZAP Express cDNA library was constructed with mRNA obtained from immature miracidia within eggs, hatched miracidia, and sporocysts of Echinostoma paraensei. This cDNA library was amplified and 213 expressed sequence tag (EST) sequences (averaging 466 nucleotides in length) were obtained. The mean percentage of unresolved bases within the EST… CONTINUE READING