Analysis of lolB gene sequence and its use in the development of a PCR assay for the detection of Vibrio cholerae.

@article{Lalitha2008AnalysisOL,
  title={Analysis of lolB gene sequence and its use in the development of a PCR assay for the detection of Vibrio cholerae.},
  author={Pattabhiraman Lalitha and Mohd Noor Siti Suraiya and Kah Leong Lim and So Yeun Lee and A R Nur Haslindawaty and Yin Yin Chan and Asma Ismail and Zainul Fadziruddin Zainuddin and Manickam Ravichandran},
  journal={Journal of microbiological methods},
  year={2008},
  volume={75 1},
  pages={142-4}
}
A PCR assay has been developed based on a lolB (hemM) gene, which was found to be highly conserved among the Vibrio cholerae species but non-conserved among the other enteric bacteria. The lolB PCR detected all O1, O139 and non-O1/non-O139 serogroup and biotypes of V. cholerae. The analytical specificity of this assay was 100% while the analytical sensitivity was 10 pg/microL and 10(3) CFU/mL at DNA and bacterial level respectively. The diagnostic sensitivity and specificity was 98.5% and 100… CONTINUE READING

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