Analysis of immunoglobulin glycosylation by LC‐ESI‐MS of glycopeptides and oligosaccharides

@article{Stadlmann2008AnalysisOI,
  title={Analysis of immunoglobulin glycosylation by LC‐ESI‐MS of glycopeptides and oligosaccharides},
  author={Johannes Stadlmann and Martin Pabst and Daniel Kolarich and Renate Kunert and Friedrich Altmann},
  journal={PROTEOMICS},
  year={2008},
  volume={8}
}
Two LC‐ESI‐MS methods for the analysis of antibody glycosylation are presented. In the first approach, tryptic glycopeptides are separated by RP chromatography and analyzed by ESI‐MS. This “glycopeptide strategy” allows a protein‐ and subclass‐specific quantitation of both neutral and sialylated glycan structures. Additional information about under‐ or deglycosylation and the protein backbone, e.g., termini, can be extracted from the same data. In the second LC‐ESI‐MS method, released… 
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