Analysis of immune complexes by two-dimensional gel electrophoresis.

  title={Analysis of immune complexes by two-dimensional gel electrophoresis.},
  author={Soprintendenza Per and John L. Abruzzo and Ralph T. Heimer},
  journal={Clinical immunology and immunopathology},
  volume={34 2},
High resolution two-dimensional (2D) gel electrophoresis is useful for analysis of constituents of immune complexes (IC) in serum, provided that the samples for the analysis are prepared by a standardized and effective purification protocol. The details of the protocol, which involve gel permeation chromatography and adsorption with protein A-Sepharose, were worked out with a model system of radiolabeled antigen bound to antibody. With this protocol one can attain an over 50% recovery of the… CONTINUE READING