Analysis of human transferrin glycopeptides by capillary electrophoresis and capillary liquid chromatography-mass spectrometry. Application to diagnosis of alcohol dependence.

@article{Barroso2013AnalysisOH,
  title={Analysis of human transferrin glycopeptides by capillary electrophoresis and capillary liquid chromatography-mass spectrometry. Application to diagnosis of alcohol dependence.},
  author={Albert Barroso and Estela Gim{\'e}nez and Fernando Benavente and Jos{\'e} M Filho Barbosa and Victoria M Sanz-Nebot},
  journal={Analytica chimica acta},
  year={2013},
  volume={804},
  pages={167-75}
}
In this study, capillary electrophoresis and capillary liquid chromatography coupled to mass spectrometry (CE-TOF-MS and μLC-TOF-MS) were used to detect and characterise human transferrin (Tf) glycopeptide glycoforms obtained by tryptic digestion. After selecting μLC-TOF-MS because of improved performance in analysis of N413 and N611 glycopeptide glycoforms, the proposed methodology was applied to serum samples. Two immunoaffinity columns were employed to isolate Tf from serum samples. Both… CONTINUE READING
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