Analysis of human IL-6 mutants expressed in Escherichia coli. Biologic activities are not affected by deletion of amino acids 1-28.

@article{Brakenhoff1989AnalysisOH,
  title={Analysis of human IL-6 mutants expressed in Escherichia coli. Biologic activities are not affected by deletion of amino acids 1-28.},
  author={Jan P. G. Brakenhoff and Melanie J. Hart and Lucien A. Aarden},
  journal={Journal of immunology},
  year={1989},
  volume={143 4},
  pages={1175-82}
}
We have constructed and analyzed amino terminally deleted analogs of IL-6. Progressively shortened variants of mature IL-6 were constructed at the cDNA level and expressed in Escherichia coli. Mutant proteins were recovered from refractile bodies by solubilizing in 6 M guanidine-HCl. The mutant protein concentration in these preparations was estimated by Western blotting by using an IL-6-specific mAb and the biologic activity was measured in the B9 (hybridoma growth factor) assay. The first 28… CONTINUE READING
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