Analysis of highly polar compounds of plant origin: combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometry.

@article{Tolstikov2002AnalysisOH,
  title={Analysis of highly polar compounds of plant origin: combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometry.},
  author={Vladimir V. Tolstikov and Oliver Fiehn},
  journal={Analytical biochemistry},
  year={2002},
  volume={301 2},
  pages={
          298-307
        }
}
The primary goal of metabolomic analysis is the unbiased relative quantification of every metabolite in a biological system. A number of different metabolite-profiling techniques must be combined to make this possible. Here we report the separation and analysis of highly polar compounds in a proof of concept study. Compounds were separated and analyzed using hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization (ESI) mass spectrometry. Two types of HILIC… Expand
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References

SHOWING 1-10 OF 29 REFERENCES
Hydrophilic interaction chromatography-electrospray mass spectrometry analysis of polar compounds for natural product drug discovery.
  • M. Strege
  • Chemistry, Medicine
  • Analytical chemistry
  • 1998
TLDR
In this investigation, amide-, Polyhydroxyethyl Aspartamide-, and cyclodextrin-based packings provided superior performance for the analysis of a set of polar natural product compounds and an optimized HILIC-ESI-MS system was successfully applied for theAnalysis of complex natural product mixtures. Expand
High-performance liquid chromatographic-electrospray ionization mass spectrometric analyses for the integration of natural products with modern high-throughput screening.
  • M. Strege
  • Chemistry, Medicine
  • Journal of chromatography. B, Biomedical sciences and applications
  • 1999
TLDR
The high resolution provided by reversed-phase HPLC coupled with the gentle and relatively universal ionization facilitated by the electrospray process has had significant impact upon a variety of procedures associated with the HTS of natural products, including extract sample diversity evaluation, dereplication, structure elucidation, preparative isolation, and affinity-based biological activity evaluation. Expand
Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry.
TLDR
More than 15 uncommon plant metabolites were identified, some of which are novel in Arabidopsis, such as tartronate semialdehyde, citramalic acid, allothreonine, or glycolic amide. Expand
Structural determination and quantitative analysis of bacterial phospholipids using liquid chromatography/electrospray ionization/mass spectrometry
Abstract This report presents a comprehensive spectral analysis of common bacterial phospholipids using electrospray/mass spectrometry (ESI/MS) under both negative and positive ionization conditions.Expand
Mixed-mode anion-cation exchange/hydrophilic interaction liquid chromatography-electrospray mass spectrometry as an alternative to reversed phase for small molecule drug discovery
TLDR
A mixed-mode anion-cation exchange/hydrophilic interaction chromatography (ACE-HILIC) method has been developed to provide both direct compatibility with ESI-MS and evaporative light-scattering detection (ELSD) and separation selectivity highly orthogonal to RP-HPLC. Expand
Hydrophilic-interaction chromatography of complex carbohydrates.
TLDR
Complex carbohydrates can frequently be separated using hydrophilic-interaction chromatography (HILIC), which is promising for a variety of analytical and preparative applications. Expand
Parameter optimization for the analysis of underivatized protein amino acids by liquid chromatography and ionspray tandem mass spectrometry.
TLDR
The analysis of the 20 underivatized protein amino acids by liquid chromatography ionspray tandem mass spectrometry is investigated in positive ion mode to obtain the maximum sensitivity for each amino acid, by using the optimum collision energy. Expand
Chromatographic separations of aromatic carboxylic acids.
  • M. Waksmundzka-hajnos
  • Chemistry, Medicine
  • Journal of chromatography. B, Biomedical sciences and applications
  • 1998
TLDR
The review describes the dependence of the retention of ionic solutes on pH and solvent composition as well as on the ionic strength of a mobile phase and principles and application of ion-exchange chromatography to the purification, isolation and less frequently, to chromatographic analysis are discussed. Expand
High-sensitivity analysis of neutral underivatized oligosaccharides by nanoelectrospray mass spectrometry.
TLDR
Nanoscale electrospray ionization (nano-ESI) overcomes the sensitivity problems found and reported for neutral oligosaccharides in conventional microscale forced-flow ESI and fully employs the MSn capabilities of an ion-trap mass spectrometer for structural analysis. Expand
Comparison of negative and positive ion electrospray tandem mass spectrometry for the liquid chromatography tandem mass spectrometry analysis of oxidized deoxynucleosides
TLDR
Investigation of the fragmentation pathways indicated some new general rules for the fragmentation of negatively charged oxidized nucleosides, and negative ion electrospray tandem mass spectrometry with CID would be preferred to positive ion mode for the analysis of sets of oxidized deoxynucleosides that include thymidine glycol. Expand
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