Analysis of glycosyl phosphatidylinositol-anchored proteins by two-dimensional gel electrophoresis.

@article{Fivaz2000AnalysisOG,
  title={Analysis of glycosyl phosphatidylinositol-anchored proteins by two-dimensional gel electrophoresis.},
  author={Marc Fivaz and Francis Vilbois and Christian Pasquali and F Gisou van der Goot},
  journal={Electrophoresis},
  year={2000},
  volume={21 16},
  pages={3351-6}
}
The aim of this study was to characterize mammalian glycosyl phosphatidylinositol (GPI)-anchored proteins y two-dimensional gel electrophoresis using immobilized pH gradients. Analysis was performed on detergent-resistant membrane fractions of baby hamster kidney (BHK) cells, since such fractions have previously been shown to be highly enriched in GPI-anchored proteins. Although the GPI-anchored proteins were readily separated by one-dimensional sodium dodecyl sulfate-polyacrylamide gel… CONTINUE READING