Analysis of glycosaminoglycans with polysaccharide lyases.

  title={Analysis of glycosaminoglycans with polysaccharide lyases.},
  author={Robert J. Linhardt},
  journal={Current protocols in molecular biology},
  volume={Chapter 17},
Polysaccharide lyases are a class of enzymes useful for analysis of glycosaminoglycans (GAGs) and the glycosaminoglycan component of proteoglycans (PGs). These enzymes cleave specific glycosidic linkages present in acidic polysaccharides and result in depolymerization. The lyases are derived from a wide variety of pathogenic and nonpathogenic bacteria and fungi. This class of enzymes includes heparin lyases (heparinases), heparan sulfate lyases (heparanases or heparitinases), chondroitin lyases… CONTINUE READING