Analysis of gene expression : northern blotting.


Messenger (m)RNAs represent the information-carrying intermediates for protein synthesis. Northern blotting gives a snapshot of the relative abundance of an RNA transcript at a set time point by measuring the steady-state levels of an RNA transcript. The steady-state level of a particular mRNA species is determined by its rate of production, degradation, and transport from the nucleus to cytoplasm. Northern blotting allows investigation and quantification of changes in gene expression.

DOI: 10.1385/1-59259-151-5:247

Cite this paper

@article{Rousell2001AnalysisOG, title={Analysis of gene expression : northern blotting.}, author={Jonathan Rousell and el-B Haddad}, journal={Methods in molecular medicine}, year={2001}, volume={56}, pages={247-61} }