Analysis of foldback sequences and repetitive sequences in cloned segments of nuclear DNA from Physarum polycephalum.

@article{McLachlan1982AnalysisOF,
  title={Analysis of foldback sequences and repetitive sequences in cloned segments of nuclear DNA from Physarum polycephalum.},
  author={Anne McLachlan and N. Hardman},
  journal={Biochimica et biophysica acta},
  year={1982},
  volume={697 1},
  pages={
          89-100
        }
}
6 Citations
Structural organization of a hypermethylated nuclear DNA component in Physarum polycephalum.
TLDR
Restriction mapping of cloned Physarum M+ DNA segments, and Southern blot analysis of genomic DNA using subcloned segments of M+DNA as a probe, provide evidence for sequence variation within different copies of the dominant highly repeated element, and possibly the other associated repeats in M- DNA.
Identification of a second retrotransposon-related element in the genome of Physarum polycephalum
SummaryThe repetitive fraction of the genome of the eukaryotic slime mould Physarum polycephalum is dominated by Tp1, a family of retrotransposon-like sequences. Tp1 elements are arranged in
Slime moulds and the origin of foldback DNA
  • N. Hardman
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1986
The genomes of the slime moulds are relatively small when compared with those of higher eukaryotes. They also contain far fewer families of repetitive sequences. Nevertheless, the general patterns of

References

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Sequence organisation in nuclear DNA from Physarum polycephalum. Interspersion of repetitive and single-copy sequences.
TLDR
Hydxyapatite binding of DNA fragments of different sizes containing reassociated repeated elements demonstrates that these sequences are interspersed with single-copy sequences in a large portion of the Physarum genome.
Sequence organisation in nuclear DNA from Physarum polycephalum: methylation of repetitive sequences.
TLDR
Comparisons of the 'fingerprint' patterns of total Physarum DNA and isolated M+ DNA after digestion using different restriction endonucleases and probing for the presence of specific repetitive sequences in Southern blots of M+ and M- DNA indicate that many repetitive sequences are shared by both compartments, though some repetitive sequences appear to be considerably enriched, or are present exclusively, either in M+.
Distribution of inverted repeat sequences in nuclear DNA from Physarum polycephalum.
TLDR
Observations suggest that the different segments of Physarum DNA from which foldback structures are derived contain nucleotide sequences that share a highly ordered and unform pattern of structural organisation.
Periodic organisation of foldback sequences in Physarum polycephalum nuclear DNA.
Nuclear DNA from the slime mould Physarum polycephalum is shown to contain interspersed inverted repeat sequences, such that denatured fragments of DNA containing pairs of these sequences form
Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 μm plasmid
A sequence of 1019 nucleotides encompassing one of the 600 base inverted repeats and non-repeated flanking regions has been determined in the type A yeast 2 micrometers plasmid cloned in pMB9.
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